How To Use It
How to get the most out of PROVEN® 40
Best-Management Practices
- Stewardship
- Replace “up to” 40 pounds of Nitrogen
- Retain In-Season Nitrogen
- Optimize pH, K, S, and other Nutrients to Maximize Nitrogen Replacement Potential
- Run Proper Trials

In-Furrow
As with any input in your operation, knowing how to use Pivot Bio microbial fertilizer is key to ensuring efficacy and product performance. Our nitrogen-producing microbes work with your crop to deliver nitrogen throughout the growing season. Because microbes are living organisms, it’s important to handle the products as instructed — whether in-furrow or on-seed — and closely follow recommended guidance on delivery, storage and use.
In-Furrow Care and Handling
Delivery, Storage and Usage
- All Pivot Bio products contain expiration dates.
- Follow all use guidelines by product.
- It is important to note that temperature affects microbe stability. Temperatures above 75-degrees F can reduce microbe stability.
- PROVEN®40 Liquid In-Furrow (LIF) product has an in-package life of up 120 days when stored properly.
- PROVEN®40 LIF must be used within 24 hours after opening.
- Keep out of direct sunlight and store between 32 degrees and 70 degrees.

In-Furrow Application Best Practices
Pivot Bio PROVEN®40 contains living microbes which will provide ammonia to corn when applied according to directions for use. This 36-pound product of Pivot Bio PROVEN®40 will treat 40 acres of corn and must be used as an in-furrow application.
Each unit of Pivot Bio PROVEN®40 may be mixed into the application tank with compatible in-furrow/”pop-up” fertilizers and insecticides at planting. For compatibility or technical questions with the liquid in-furrow application, contact your regional representative.
PROVEN®40 In-furrow Safety Data Sheet
Application Best Practices
Tank Mix Calculator
Use the calculator to determine your mixing ratios. There are two tank mix calculation methods below. Either may be useful to you depending on your situation at the time.
Tank Mix Calculator
On-Seed
As with any input in your operation, knowing how to use Pivot Bio microbial fertilizer is key to ensuring efficacy and product performance. Our nitrogen-producing microbes work with your crop to deliver nitrogen throughout the growing season. Because microbes are living organisms, it’s important to handle the products as instructed — whether in-furrow or on-seed — and closely follow recommended guidance on delivery, storage and use.
On-Seed Care and Handling
Delivery, Storage and Usage
- All Pivot Bio products contain expiration dates.
- Follow all use guidelines by product.
- It is important to note that temperature affects microbe stability. Temperatures above 75-degrees F can reduce microbe stability.
- PROVEN®40 OS has an on-seed stability of up to 60 days.
- PROVEN®40 OS is most stable on seed between 32- and 70-degrees F.
- PROVEN®40 OS microbe stability can be impacted by temperatures above 75-degrees F.

On-Seed Application Best Practices
Pivot Bio PROVEN®40 OS contains living microbes which will provide ammonia to corn when applied according to directions for use. This 4.25L product of Pivot Bio PROVEN®40 OS will treat one ProBox (50 units of seed) as a seed treatment.
Learn more about the best ways to ensure efficacy and product performance when using Pivot Bio PROVEN 40 On-Seed.
PROVEN®40 On-Seed Safety Data Sheet
Application Best Practices
PROVEN®40 On-Seed Compatibility
Application of other products when applying Pivot Bio PROVEN®40 OS can result in reduced microbe viability on-seed and may impact product performance.
Please consult your local Pivot Bio Commercial Agronomist
On Seed Compatibility Sheet
PROVEN®40 On Seed Viability in Varying Soil Conditions
Develop and analyze a laboratory test to understand how varying soil conditions at planting, post planting, and up through germination can impact the viability of on-seed Proven 40 microbes.
On Seed Viability Sheet
What It Is
Pivot Bio’s gene-edited, nitrogen-fixing microbes represent the first new mode of action in nitrogen delivery to crops this century.
Learn MoreHow It Works
Learn how Pivot Bio PROVEN 40 gene-edited and patented microbes deliver nitrogen directly to plant roots in the soil.
Learn MoreField-Tested Performance Data
Nitrogen’s Next Mode of Action – Peer Reviewed and Farmer Focused
Learn MoreProven Product Resouce Center
Here you will find information, tools and resources to understand what Pivot Bio PROVEN 40 is, how it works and how to most effectively use it to optimize yields in your fields.